Bacterial transformation lab report, sample of homeworks. Conceptual approaches to biology for majors i bio 281. In the transformation lab, we discovered the process of bacterial genetic transformation and how to calculate transformation efficiency. Biology is brought to you with support from the our mission is to provide a free, worldclass education to anyone, anywhere. Bacterial transformation lab by lindsay young on prezi. They span the globe in their topics and their techniques. Bacterial transformation some bacteria have another method of transferring dna and producing recombinants that does not require conjugation. Answers are provided in the instructors answer guide. For more information, visit this video demonstrates how to perform a bacterial. Our results supported our hypothesis and the transformation was successful.
Students transform bacterial cells with a novel gene that. Ap biologyinvestigation 8bacterial transformation lab. Genetic transformation is a process by which the genetic material carried by an individual cell is altered by the incorporation of foreign exogenous dna into its genome medicinenet, 1999. Education center k12 lessons and laboratories classroom activities in plant biotechnology. Open the tubes and using a 100 l micropipette with a sterile tip, transfer 250 l of the ice cold transformation solution cacl 2. Ap bio pglo transformation formal lab report, sample of. Ap bio pglo transformation formal lab report, sample of essays. Working in teams, each team uses an unidentified plasmid that is either kanamycinresistant or ampicillinresistant and could possibly also code for the gene for green fluorescent protein gfp. Biology is brought to you with support from the amgen foundation. Thus you will see only individual colonies on the plate. This video demonstrates how to perform a bacterial transformation using the biorad pglo bacterial transformation kit. From the previous lab, we can identify our plasmids.
Thursday quiz on plants, begin lecture on immune system, using personal notes, not book friday complete lecture and notes on immune system. Since only some of the cells exposed to the amp r plasmids will actually take them in, only some cells will be transformed. The progression from dna rna protein trait is shown with the bacteria e. Bacterial transformation lab ap biology heather swain dp. In molecular biology, transformation refers to a form of genetic exchange in. See more ideas about bacterial transformation, ap biology and microbiology.
The pglolb control plate can be compared to any of the lbamp plates to show that plasmid uptake is required for the growth in the presence. Concepts you will need to know include identifying a word to describe. Classic bacterial transformation lab using pglo plasmid from biorad. He explains how you can use the pglo plasmid to produce glowing e. The plasmid pglo contains an antibioticresistance gene, ampr, and the gfp gene is regulated by the control region of the ara operon.
For ap biology, your participation in lab is an integral part of the class experience as well as your grade 40%. We have kits for new and traditional ap biology labs. The labs in this manual support the concepts, content, and science practices within the revised ap biology course. The pglo plasmid contained a gene for the green fluorescent protein gfp from a bioluminescent jellyfish and a gene for resistance to ampicillin, an antibiotic. More specifically, a previously prepared pglo plasmidwhich consisted of the gene to be clonedwas used to transform nonpathogenic bacteria. Its going to be my first year teaching ap bio, so your videos are very. Escherichia coli are commensal gram negative bacteria found in the guts of humans. Nathan smith abstract bacterial transformation is the easiest form because bacteria is a singlecelled organism, therefore happens faster and has a higher success rate of genetic transformation. Genetic transformation is an active uptake of free dna by a bacterial cell like e. Bacterial transformation lab report laura downing dp. Bacterial transformation lab ap biology during this bacterial transformation lab, we inserted a plasmid containing a antibioticresistance gene. Bacterial transformation is of central importance in molecular biology. One method to achieve this is through chemical competence with heat shock.
Oct 16, 2012 this video demonstrates how to perform a bacterial transformation using the biorad pglo bacterial transformation kit. Mar 26, 2015 classic bacterial transformation lab using pglo plasmid from biorad. Information for teacherssafety instructions although the e. They have the capacity to double every twenty minutes and make a favorable carrier of recombinant dna. Their genetic information is encoded in one large chromosome in addition to plasmids, which are small, circular molecules of dna campbell and reece 2005. The art of bacterial transformation introduction purpose.
Transformation lab a plasmid discovery labratory 6, ap biology abstract. Collectively, they created ap biology investigative labs. Pearson, as an active contributor to the biology learning community, is pleased to provide free access to the classic edition of the biology place to all educators and their students. Write a hypothesis that reflects the experiment and predict experi. Transforming li strains with green fluorescent protein. Also, it could be used to see what other genes can be turned on or off. This comparison shows that genetic transformation produces bacterial colonies that can grow on ampicillin due to the uptake of the pglo plasmid and the expression of the ampicillin resistance gene. The lbamp pglo plate will have no growth, the lb pglo plate will have a lawn of growth meaning colonies covering. View lab report 3867838apbiologytransformationlabreport from microbiolo at national university of singapore. To study the behavior of escherichia coli once it has been introduced to a foreign gene. Describe plasmid uptake and how transformation is determined 6 points maximum. The pglo bacterial transformation lab is based off the molecular biology ap biology lab involving transformation.
Connect concepts with techniques and put them into context with. If the ampicillin resistant dna is introduced into the e. Bacterial transformation, the process in which a plasmid is induced into a bacterial host, is one example of genetic engineering, which is any humancreated changes in an organisms dna. We enable science by offering product choice, services, process excellence and our people make it happen. In the bacterial transformation advanced inquiry lab kit for ap biology, study biotechnology, which raises a lot of questions about the ethics of modifying. The conversion of one genotype into another by the introduction of exogenous dna that is, bits of dna from an external source is termed transformation. Address and record the following in your laboratory notebook or on a. The purpose of this lab was to discover whether or not one could.
Investigation 8 bacterial plasmidbased genetic transformation, enables students to manipulate genetic information in a. This web page was produced as an assignment for an ap biology course at montgomery. Use this worksheet and quiz to quantify your knowledge of a bacterial transformation biology lab. This gene transfer is accomplished with the aid of a plasmid, a naturallyoccuring small piece of circular dna in bacteria. Essentially, genetic transformation involves the process where a cell in this lab, a bacterial cell takes up foreign dna from its surroundings and incorporates it into its own dna. Ap biology bacterial transformation lab by kenneth. Summarize the overall idea of the procedures in a few steps. Transformation was discovered in streptococcus pneumoniae in 1928 by frederick griffith. When lab is complete, collect all petri dishes, open, and immerse in a 10%. Choose from our kits, follow a college board lab, or design your own with our wide variety of equipment and supplies. Ravesiweinstein science coordinator attleboro high school 100 rathbun willard dr. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Ap biologyinvestigation 8bacterial transformation lab walkthrough.
Dec 17, 2014 transformation lab a plasmid discovery labratory 6, ap biology abstract. Dna on plate 1 since there is no antibiotic present the wildtype bacteria grew normally and formed a lawn across the whole plate. Bacterial transformation an introduction to genetic. See science manual bacterial transformation lab for complete list of materials and procedures.
During this bacterial transformation lab, we inserted a plasmid containing a. Introduction bacteria are microscopic, singlecelled organisms. Answer the lab packet questions as you proceed through the virtual lab. The purpose of this lab is to insert genes that will make e. Open the tubes and using a 100 l micropipette with a sterile tip, transfer 250 l of the ice cold transformation solution cacl. Select lab 6 molecular biology part i transformation in bacteria 3. Prepare your students for medical and lab tech careers with carolina s wide range of equipment, kits and models. Even though we referenced the diagram in our lab manual that showed how much. Read the lab safety information and write the complete procedure pp. Next generation science standards and the newest ap biology materials support. We followed the instructions listed in the carolina transformation for ap biology. Bacterial transformation lab report bio 281 asu studocu.
Transformation defined process of transformation of a plasmid isolation isolate plasmidsagar plate that grows only colonies of resistance gene antibiotic use of antibiotic resistancesensitivity genes detailed description of antibiotic resistance lab procedure gel electrophoresis isolate. Introduction abstract our alternate hypothesis for this experiment will be that the pglo dna will incorporate in the e. Coli bacterial cells through the transformation process carolina biological supply co. Since 1989, there have been 12 laboratorieslovingly nicknamed the dirty dozenthat are suggested curricula for ap biology classes. Cold spring harbor laboratorys dna learning center presents this course as a service to help engage teachers and students in china during. Ap biology investigation 8 bacterial transformation lab walkthrough. Wednesday ap biology lab 9 transpiration formal report due on april 8 thursday quiz on plants, begin lecture on immune system, using personal notes, not book friday complete lecture and notes on immune system. Bacterial transformation lab report free essay example. Bacterial transformation advanced student laboratory kit. Big genetics and information transfer 3 ap central. Only transformed cells can grow on agar with ampicillin. Duplication of any part of this document is permitted for classroom use only. In lab groups, we genetically engineered the bacteria with pglo plasmids containing specific codes for gfp green fluorescent protein and ampicillin, an antibiotic, resistance.
All in all, the lab was fun and a success in transforming the bacteria, ecoli, into one that is antibiotic resistant and into one that can glow. Some bacteria have another method of transferring dna and producing recombinants that does not require conjugation. Bacterial transformation is what happens when a bacterial cell accepts outside dna and incorporates it into its own dna, something that generally happens with plasmids. New plasmid engineering and bacterial transformation highly motivated ap biology and research classes only students must have completed living environment this is a rigorous laboratory experience suitable for highly motivated students in ap biology or a research class with strong, independent laboratory skills. Topic description 1 point each transformation defined process of transformation of a plasmid isolation isolate plasmidsagar plate that grows only colonies of resistance gene antibiotic use of antibiotic resistancesensitivity genes. For technical service call your local biorad office or in the u. The answers will vary but should be in the range of 103 104 transformantsg dna. Prepare your students for medical and lab tech careers with carolina s wide range of. The goal of the lab was to identify the plasmids because it was unknown which plasmids contained which genes. Stay connected to your students with prezi video, now in microsoft teams.
In the transformation lab designed by the carolina biological supply co. The plasmids are either pglo, puc1819 or puc1819 with a 6kb insert disrupting the lacz gene. In this investigation, students will first acquire the tools to transform e. Transformation lab report biol 1100 csula studocu bacterial transformation the pglo system catalog number 1660003edu. View lab report 3867838 ap biology transformation lab report from microbiolo at national university of singapore. This report analyzes an experiment conducted in order to transform e.
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